|
KMID : 0861020130280030017
|
|
Korea Journal of Herbology
2013 Volume.28 No. 3 p.17 ~ p.24
|
|
|
Single Oral Dose Toxicity Test of Persicae Semen Aqueous Extracts in Mice
|
|
:Cho Hun-Bum
:Park Ji-Ha/:Seo Bu-Il/:Cho Su-Yeon/:Park Kyu-Ryul/:Choi Seung-Hoon/:Han Chang-Kyun/:Song Chang-Hyun/:Park Soo-Jin/:Ku Sae-Kwang
|
|
|
Abstract
|
|
|
Objectives : This study was to evaluate the single dose toxicity of Persicae Semen (PS) in ICR mice.
Methods : Aqueous extracts of PS (Yield = 18.60%) were administered as an oral dose of 2,000, 1,000 and 500 mg/kg (body weight) according to the recommendation of Korea Food and Drug Administration (KFDA) guidelines (2009-116, 2009). Animals were monitored for the mortality and changes in body weight, clinical signs and gross observation during 14 days after dosing, upon necropsy; organ weight and histopathology of 12 principle organs were examined.
Results : Amygdalin contents in PS aqueous extracts were detected as 32.50±5.96 μg/ml. We could not find any PS extracts treatment related mortalities, clinical signs, changes on the body and organ weights, gross and histopathological observations up to 2,000 mg/kg in both female and male mice, except for transient and slight loss of locomotion detected in female and male mice treated with 2,000 mg/kg. In addition, pharmacological immunomodulatory effects related findings were also demonstrated in 2,000mg/kg treated female and male mice as hypertrophy and hyperplasia of lymphoid cells in the submandibular lymph nodes.
Conclusions : Based on the results of this experiment, the approximate lethal dose (ALD) of PS extracts after single oral treatment in female and male mice were considered above 2,000 mg/kg, respectively. It should be carefully used in clinics because the possibilities of respiratory or neurological disorders were observed when administered over 2,000 mg/kg of PS extract related to amygdalin.
|
|
|
KEYWORD
|
|
|
Persicae Semen, Single oral dose toxicity, Mouse, Amygdalin, Histopathology
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|